摘要
目的 :比较中国人群 和 型丙型肝炎病毒 (HCV)包膜蛋白 E2高变区 1(HVR1)序列变异特点 ,并探讨其意义。方法 :应用逆转录巢式 PCR技术从 5 5例中国人 型、38例中国人 型 HCV感染患者血清中扩增 HCV部分包膜区基因片段[核苷酸 (nt) 144 9～ 15 86 (HCV- J)或 nt 146 0～ 15 82 (HCV - J6 ) ],纯化后直接采用双脱氧链末端终止法进行序列分析。 结果 :中国人群 、 型 HCV HVR1均位于氨基酸 (aa) 384～ 40 8位 ,内有 5个相同的保守位点 :385位 Thr,389、390、40 6位Gly,40 3位 Phe。此外 ,在 型 HVR1中 40 1位 Ser也相当保守。 、 型间变异特点相似 ,但部分位点的变异程度、氨基酸出现的种类与频率及高变区两端保守区序列仍有差异。 结论 :对 HVR1序列变异规律及其生物学意义的进一步研究将有助于了解
Objective: To compare the sequence diversity of HVR1 in the putative envelope protein E2 of the genotypeⅡand genotype Ⅲ HCV in Chinese. Methods: The cDNAs[nucleotide(nt)1449-1586(HCV-J) or nt1460-1582(HCV-J6)] derived from plasma of 55 patients infected with genotype Ⅱ HCV and 38 patients infected with genotype Ⅲ HCV were amplified,purified and directly sequenced by RT-nested polymerase chain reaction(PCR) and dideoxynucleotide chain termination method. Results: The HVR1 was found in amino acid(aa) 384-408 positions of both types HCV E2 protein. There were 5 similar conserved amino acids in 2 types HCV HVR1:aa385(Thr), aa389, 390, 406(Gly)and aa403(Phe).Besides, 401(Ser) was also highly conserved in genotype Ⅱ HCV HVR1. Although the variation characteristic of 2 types was similar, but the sequence diversity(SD),the kinds and frequency of some amino acids in some HVR1 positions and the conserved region near the HVR1 had some differences between 2 genotypes. Conclusion: Further study on the diversity of HVR1 and its biological significance will be helpful to understand the mechanism of HCV persistent infection and the development of HCV vaccine.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第9期852-854,共3页
Academic Journal of Second Military Medical University
基金
全军九五课题基金资助项目 ( 96 M174
98Z0 89)
关键词
丙型肝炎病毒
高变区1
序列分析
遗传学
变异规律
hepatitis C virus
hypervariable region 1
sequence analysis
variation(genetics)
