摘要
观察浅低温体外循环 (CPB)心内直视手术中 ,颈内静脉血S - 10 0蛋白和NSE的含量变化及其对脑损伤诊断的临床意义。择期心内直视手术患者共 15例 ,于CPB开始前 (A点 )以及CPB开始后 ,分别于鼻咽温度(NPT)降温稳定期 (B点 )、复温至NPT36℃ (C点 ) ,同时在CPB结束后分别于 30min(D点 )、4- 5h(E点 )以及2 4h(F点 ) ,经左颈内静脉球部取血样 ,用化学发光免疫分析方法测定血浆中S - 10 0蛋白含量 ,放射免疫双抗体夹心法测定血浆NSE含量。结果表明 :CPB开始后S - 10 0蛋白和NSE随着NPT的下降而升高 ;在CPB结束时 ,S - 10 0蛋白的水平达最高值 ,NSE则在CPB结束后 30min达到最大值 ,然后随NPT的恢复而下降 ,2 4h后 ,S - 10 0蛋白恢复到术前的水平 ,NSE仍高于术前水平 (P <0 .0 5 ) ,但仍属正常范围。总之CPB可引起S - 10 0蛋白和NSE的释放 ,提示有脑损伤发生 ,但损伤是可逆的 ,在CPB停止后 。
To observe dynamic changes of S-100 protein and NSE during mild hypothermia cardiopulmonary bypass(CPB), the venous blood samples of 25 patients with elective cardiac surgery were obtained simultaneously from the left artery and left jugular bulb before CPB(A), hypothermia periold(32-35℃)(B) and rewarming to 36℃(C) during CPB, 30 minutes(D), 4-6 hours(E) and 24 hours(F) after CPB. Plasma S-100 protein concentration was determined by chemilumunescence immunoassay, and NSE level was determined by radioimmunoassay. The results showed that the levels of S-100 protein and NSE increased significantly during CPB, and NSE peaked at 30 minutes(D) after CPB. It suggested the central nervous system dysfunctions. The S-100 protein and NSE concentrations decreased gradually and retuned to normal nearly(F) after mild hypothermia CPB. It suggested that there were not obvious central nervous system dysfunctions.
出处
《标记免疫分析与临床》
CAS
2001年第3期149-151,共3页
Labeled Immunoassays and Clinical Medicine
