摘要
目的 :分析研究该室保存或构建的各类痢疾菌毒株、减毒突变株、菌苗株的毒力及其生物表型的相互关系 ,探讨与致病有关的成分 ,为改进现有菌苗和构建新型菌苗提供参考依据。方法 :采用刚果红结合试验、接触性溶血试验、HeLa细胞入侵试验及豚鼠角结合膜炎试验 ,对不同痢疾菌的毒力表型进行检测 ,并对大质粒及其侵袭相关的基因 ( 4 1kb)进行遗传背景分析。用BA immunoblot法 ,对痢疾杆菌和侵袭性大肠杆菌等进行毒力相关抗原的分析。结果 :有侵袭毒力的痢疾菌株均与刚果红染料结合、有接触性溶血活性 ,可侵入HeLa细胞 ,豚鼠角结合膜试验阳性 ,并都含有大质粒 ( 12 0～ 14 0MD) ,与 4 1kb侵袭探针杂交也均呈阳性反应 ;而无毒力的痢疾菌株上述生物表型均为阴性。有毒痢疾菌和侵袭性大肠杆菌均表达 4种主要毒力相关抗原Ipa(A ,B ,C ,D) ,而无毒株不表达这些抗原。痢疾菌的毒力不仅与Ipa有关 ,而且还与LPS抗原有关。结论 :细菌的毒力与生物表型密切相关。并且Ipa与LPS这两类抗原均表达时细菌才有毒力。
Objective:To analyze the relationship between virulent phenotypes and the expression of antigens.Methods:The authors observed virulent phenotypes of different Shigella and its mutant by Congo red test(Pcr),contacting hemolysis test (CHT),invading HeLa cells test(Inv) and keratoconjunctivits test(Ser) and analyzed genetic background of the virulence of Shigella.Took the convalescent sera of monkeys infected with Shigella flexneri 2a to analyze the virulence associated antigens of different Shigella and enteroinvasive E.coli2 by BA immunoblot methods.Results:All virulent Shigella strains have a 120～140 MegaDalton(MD) plasmid and are Pcr(+),CHT(+),Inv(+),Ser(+).While all virulent Shigella strains are negative.All virulent strains contain four invasive plasmid antigens(IpaA?B?C?D),while nor do virulent strains.The virulence of Shigella is related not only with Ipas,but also with LPS antigen.Conclusion:The virulence of Shigella is associate with biology phenotypes of Shigella.Only with common expression of the two antigens(Ipas and LPS),the bacteria express the virulence.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第11期571-574,共4页
Chinese Journal of Immunology
基金
国家 8 63项目经费资助 (No .10 2 0 7 0 3 0 3)
