摘要
目的 应用间期荧光原位杂交 (FISH)技术检测骨髓增生异常综合征 (MDS)患者 8号染色体三体 (8三体 )的发生率。方法 采用荧光素SpectrumRed直接标记 8号染色体着丝粒DNA探针 ,对 6 6例MDS患者 (病例具有连续性 )和 10例正常人的骨髓细胞进行间期FISH检测 ,并与常规细胞遗传学 (CC)结果相比较。结果 6 6例MDS患者中 ,间期FISH检测 2 3例 (34 8% )为阳性 ,而CC检测只发现其中 15例为阳性。结论 间期FISH在检测MDS患者 8三体方面十分有用 。
Objective To investigate the frequence of trisomy 8 in myelodysplastic syndromes (MDS) by interphase fluorescence in situ hybridization (FISH).Methods 66 consecutive cases of MDS and 10 normal controls were studied by interphase FISH using SpectrumRed directedly labelled chromosome 8 centromere specific DNA probe and the results were compared to those of conventional cytogenetics(CC).Results In 66 cases of MDS,23 (34 8%) were positive by interphase FISH,however,of whom,only 15 cases by CC.Conclusion Interphase FISH is very useful in detecting trisomy 8 in MDS and it is an important complement to CC.
出处
《江苏医药》
CAS
CSCD
北大核心
2001年第10期740-742,F002,共4页
Jiangsu Medical Journal
关键词
骨髓增生异常综合征
荧光原位杂交
染色体8号
细胞遗传学
Myelodysplastic syndromes Fluorescence in situ hybridization Interphase Chromosome 8 Cytogenetics
