摘要
在PHA(1μg/ml)刺激下,IL-1或TNF均能诱发静止T细胞发生增殖反应,其最适浓度为IL-1(50U/ml)和TNF(200U/ml)。利用其最适浓度,联合作用于PHA刺激T细胞,其增殖反应增强了25％,但仍低于PHA联合10％的Mφm所诱发的增殖反应。单克隆抗体anti-Tac(p55)完全阻断了由PHA联合IL-1所诱发的增殖反应,但对PHA联合TNF所诱发的增殖反应只能部份地抑制。这些结果表明,TNF除了同IL-1一样具有通过IL-2途径来诱导T细胞活化外,还有着其他的途径,提示T细胞活化增殖的第二信号可由多种单核因子提供,而最大的增殖反应的发生,则是多种单核因子和其他因素协同作用的结果。
We investigated the synergetic effect of IL-1 and TNF on the proli-ferative response of PHA-stimulated T cells. IL-1 or TNF induced the proliferative response of PHA-stimulated T cells, with optimal concentration of 50U/ml and 200U/ ml, respectively. The proliferative response was enhenced to 25% by the optimal concentration of IL-1 in combination with TNF, but lower than that induced by PHA with 10% Mφm. Anti-Tac (p55) McAb completely inhibited the proliferation induced by PHA with IL-1, but partially inhibited the proliferation induced by PHA with TNF. Our data indicate that TNF activate T cells by IL-2 dependent pathway like IL-1, as well as the pathway different from IL-2, suggesting that various monokines act as the second signals for T cell proliferation and that the maximum activation and proliferation of T cell is due to the synergetic effect of monokines and other factors.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1991年第4期216-219,共4页
Immunological Journal
关键词
白细胞介素1
肿瘤坏死因子
T细胞
Interleukine 1 (IL-1) , Tumor necrosis factor (TNF) , Monokine, Mitomycin-treated M (Mφm)
