摘要
用亲和层析技术自人脑提取脑抗原并免疫家兔制成兔抗人脑抗体,建立ELISA试验。检测结果表明:脑梗塞(0.57±0.22)、脑出血(0.38±0.19)、精神分裂症(0.38±0.13)、高血压病(0.36±0.17)和脑肿瘤(0.33±0.11)等疾病患者血清中抗脑抗体(ABAb)含量均较正常人(0.22±0.06)明显升高(P<0.001)。提示:血清中出现ABAb反映了血脑屏障受损且可能有助于了解高血压病患者脑血管病变的程度。
Antibrain serum was obtained by immunizing rabbit with brain antigen which was isolated from human brain by affinity chromatography,the ELISA for detecting ABAb (antibrain antibody) in serum was established. The results showed that the level of ABAb in serum of patients with brain infarction (0.57±0.13) , brain hemorrhage (0.38 ± 0.19) , schizophrenia (0.38±0.13) , hypertension (0.36±0.17) and brain tumor (0.33±0,11) were all significantly higher than that of normal subjects (0.22±0.06) (P<0.001) . This study suggested that ABAb in serum indicated blood brain barrier damage and may be helpful to know the degree of cerebrovascular disease in patients with hypertension.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1991年第1期45-47,共3页
Immunological Journal
关键词
抗脑抗体
自体抗体
ELISA
Brain infarction, Brain hemorrhage, Hypertension, Antibrain antibody, Autoantibody
