摘要
目的 :研究Na+ /H+ 交换抑制剂HOE6 42 (cariporide)对未成熟心肌缺血 /再灌注损伤的保护作用。方法 :取健康新西兰幼兔 (2～ 3周龄 )心脏 2 4枚 ,随机均分为 2组。对照组St ThomasⅡ液作为保护液。实验组HOE6 42 (10 μmol/L)加St ThomasⅡ液作为保护液。使用Langendorff离体心灌注实验模型 ,经受常温缺血 6 0min ,期间每隔 2 0min灌注一次心肌保护液 ,恢复再灌注后 ,观察血流动力学变化 ,并测定心功能指标。结果 :HOE6 42实验组 ,平均动脉压 (MAP) ,主动脉流量 (AF) ,冠脉流量 (CAF) ,左室收缩 /舒张压 (LVSP/LVDP)及左室压力微分 (dp/dt)恢复率明显优于单纯用St ThomasⅡ的对照组 (P <0 0 1) ,左房压 (LAP) ,HOE6 42组明显低于对照组 (P <0 0 5 )。恢复灌注后 ,HOE6 42实验组室颤发生率明显低于对照组 (P <0 0 1)。肌酸激酶 (CK)、乳酸脱氢酶 (LDH)漏出量(U/L) ,HOE6 42组明显少于单用St ThomasⅡ液组 (P <0 0 1)。结论 :HOE6 42 (cariporide)对未成熟心肌缺血
Objective:The purpose of the present study is to investigate the protective effects of HOE 642 (cariporide) on immature rabbit heart Methods: Twenty four New Zealand rabbits (2to3 weeks old) were randomly divided into two groups ( n =12, each group) Isolated working rabbit heart model was subjected to this study The hearts were perfused in a Langendorff apparatus and submitted to 60 minutes of normothermic ischemia with cardioplegia per 20 minutes and 25minutes of reperfusion Group I received St Thomas Hospital cardioplegic solution No2 as cardioprotective solution Group II received the same cardioplegic solution as group I with addition of cariporide (10μmol/l) The left ventricular function was recorded, which included left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP), coronary artery flow(CAF), mean aortic pressure(MAP), aortic flow (AF) and dp/dt max The levels ofcreatine phosphokinase (CK), lactic dehydrogenase (LDH) of coronary sinus venous solution were measured Results: Before myocardial ischemia, there were no significant differences in all above mentioned parameters in two groups But after reperfusion thepercentage of recovery of myocardial function in group II was much better than group I, the LVDP?LVSP?MAP?AF?CAF and dp/dt max showed markedly better recovery in group II The release of CK, LDH was significantly increased in group I ( P <0 01) Conclusions: Hoe 642(cariporide) with St Thomas Hospital No 2 solution can provide much better myocardial function recovery for immature myocardium in ischemia/reperfusion than St Thomas hospital No 2 solution only
出处
《军医进修学院学报》
CAS
2001年第3期202-204,共3页
Academic Journal of Pla Postgraduate Medical School
基金
回国人员启动基金项目 ( 10 0 94A)
