摘要
目的 :探讨p16、cyclinD1、CDK4 蛋白在食管鳞癌发生中的意义及其相互间的作用。方法 :采用LSAB免疫组化染色法 ,对 43例食管癌手术标本的p16、cyclinD1、CDK4 蛋白进行标记。结果 :在鳞癌组织中 ,3种抗体的阳性物质存在于细胞核中和 /或胞浆中 ,且从正常鳞状上皮→癌旁不典型增生→鳞癌组织 ,p16的阳性率逐渐下降 ,而cy clinD1、CDK4 的阳性率却逐渐上升。正常上皮与癌组织之间的阳性率差异均具有显著性 (P <0 .0 5 ) ;随着癌分化程度下降 ,p16阳性率呈下降趋势 ,III级与I级鳞癌之间阳性率差异有显著性 (P <0 .0 5 ) ,但未检出cyclinD1、CDK4 与癌分化程度的关系。结论 :p16、cyclinD1、CDK4 蛋白表达的变化是食管癌发生中的早期事件 。
Aim: To study the role of p16, cyclinD 1 and CDK 4 protein in the carcinogenesis of esophageal cancer, as well as the interaction between them. Method: p16,cyclinD 1 and CDK 4 of the specimens from 43 case of esophageal squamous cell cancer were labeled by immunohistochemical LSAB method. Results: The positive substance of p16,cyclinD 1 and CDK 4 were all found in nucleus and cytoplasm in cancer tissue; from the normal epithelia to displasia to squamous cancer tissue, the positivity rates of p16 decreased gradually, while those of cyclinD 1 and CDK 4 increased. There were significant differences in positivity rate of p16,cyclinD 1 and CDK 4 between the cancer tissue and the normal epithelia ( P <0.05),meanwhile, the positivity rates of p16 has significant difference between cancer tissues at grate Ⅲ and grate Ⅰ, but there were no significant differences in positivity rates of cyclinD 1 and CDK 4 between each grade. Conclusion:The study demonstrates that the alterations of p16, cyclinD 1 and CDK 4 protein are an early event in the carcinogenesis of the esophagus cancer, in addition, the disfunction of modification pathway which they made up of may involve in the carcinogenesis of esophageal squamous cell carcinoma.
出处
《河南医科大学学报》
北大核心
2001年第5期539-541,共3页
Journal of Henan Medical University
基金
河南省重大科技攻关资助项目 ( 96 10 0 1)
