摘要
用免疫组化法和流式细胞仪对肿瘤细胞系MCF 7和MCF 7 Adr的P 糖蛋白表达水平进行定性定量研究。用噻唑蓝比色法 (MTT)研究茶多酚的细胞毒性及其对耐药性的逆转作用 ,并与Pgp抑制剂———奎尼定进行了比较。免疫组化法检测P 糖蛋白表达水平 ,MCF 7 Adr呈强阳性 ,而MCF 7呈阴性 ;流式细胞仪定量检测结果MCF 7 Adr细胞系细胞阳性率为 15 % ,MCF 7细胞系细胞阳性率为 1 8%。MCF 7 Adr细胞系存在Pgp的过度表达。噻唑蓝比色法 (MTT)检测结果茶多酚、奎尼定的加入对阿霉素对MCF 7的细胞毒性几乎没有影响。而茶多酚、奎尼定的加入明显增加了MCF 7 Adr对阿霉素的敏感性。免疫组化与流式细胞技术结合 ,可用于肿瘤细胞系P 糖蛋白表达水平的定性定量检测。茶多酚不仅具有一定的抗癌活性 ,还与奎尼定一样具有多药耐药性逆转作用。
MDR1 express product P glycoprotein was detected by immunocytochemical method and flowcytometry.The cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by MTT assay in MCF 7 and MCF 7/Adr carcinoma cell lines,and compared with Pgp inhibitor quinidine.The Pgp expression of MCF 7/Adr was strongly positive,the positive rate was 15%;the Pgp expression of MCF 7 was negative,the positive rate was 1.8%.IC50 of tea polyphenol to MCF 7 and MCF 7/Adr is 115.2?μg/mL and 207.6?μg/mL respectively.IC50 of quinidine to MCF 7 and MCF 7/Adr is 129.8?μmol/L(42.1?μg/mL)and 94.1?μmol/L(30.5?μg/mL)respectively.Tea polyphenol and quinidine changed little toxicity of adriamycin to MCF 7,but tea polyphenol and quinidine improved the sensitivity of MCF 7Adr to adriamycin significantly.Immunocytochemistry and flow cytometry can detect P glycoprotein expression level qualitatively and quantitatively.Tea polyphenol is not only an anti tumor agent,but also a multidrug resistant modulator similar as quinidine.Tea polyphenol is advantageous for its little toxicity in tumor treatment.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第4期496-501,共6页
Acta Scientiarum Naturalium Universitatis Pekinensis
基金
国家自然科学基金资助项目 ( 94 73 10 2 0 0 2 )
