摘要
目的 :了解河北省某恙虫病疫源地鼠宿主的感染状况 ,确定恙虫病传播宿主。方法 :捕捉疫村内及疫村周围鼠 ,采集血标本检测恙虫病立克次体的荧光抗体 ,解剖观察肝脾肿大情况 ,采集肝脾标本接种昆明种小白鼠 ,观察传代鼠的发病情况 ,涂片或切片检测其肝脾中恙虫病立克次体 ,检测其血清中的荧光抗体。结果 :不同鼠肝脾肿大率为 6 0 %～ 10 0 % ,鼠的恙虫病抗体流行率为 0 %～ 5 0 % ,传代鼠的发病率为 96 .2 3%～ 10 0 % ,传代鼠的肝脾涂片Rt阳性率为 2 5 %～ 5 0 % ,仅在褐家鼠肝脾的传代鼠血清中查到抗体 ,阳性率为 9.5 2 %。 10份肝脾切片中 9份检出Rt。结论 :宅区褐家鼠、野外大仓鼠和黑线姬鼠为当地恙虫病传播宿主。
Objective: To study infection state of Richettsia tsutsugamushi (Rt) among mouse host in epidemic focus in Hebei province. Methods: Following items were carried out:to catch mice in epidemic village and field (catch-mouse), observe abnormal phenomena of liver and spleen, inoculate liver and spleen samples to small white mouse with Kunming species (experimental mouse), smear and/or freeze cut section of liver and spleen from experimental mouse to detect Rt, take blood specimens from catch-mouse and experimental mouse to test antibodies against Rt with IFA. Results: In the different species mice caught, the rate of hepatomegaly and/or splenomegaly were from 60% to 100%,and prevalance of Rt antibody were from 0% to 50%. In the experimental mouse with inoculation liver and spleen samples came from different species mice caught, incidence of the disease were from 96.23% to 100%, smear positive rate of Rt were 25% to 50%, 9 of 10 freezing cut section samples were Rt positive, the positive rate of Rt antibodies of experimental mouse sera came from Rattus norvegicusit was 9.52 %. Conclusion: Transmissible hosts in the residence area are Rattus norvegicusit , and in the field are Cricetulus triton and Apodemus agrarius .
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2001年第5期365-368,共4页
Chinese Journal of Vector Biology and Control
基金
河北省科委立项课题
