摘要
目的 探讨甲基四氢叶酸还原酶 (methylenetetrahydrofolate reductase,MTHFR)基因多态性与糖尿病肾病 (diabetic nephropathy,DN)发生的关系。方法 利用聚合酶链反应 -限制性片段长度多态性分析法 (PCR- RFL P)检测了 85名健康人和经尿微量白蛋白检测确诊的 82例合并 DN和 79例无 DN的2型糖尿病患者 MTHFR基因第 6 77位碱基多态性。结果 DN患者 MTHFR基因变异型纯合子和等位基因频率均明显高于无肾病患者及健康者 ,P<0 .0 1。结论 MTHFR基因第 6 77位碱基变异可能是中国汉族人
Objective: To investigate the relationship between methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and diabetic nephropathy (DN). Methods: The technology of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect MTHFR gene C677T mutation in 85 healthy controls, 79 type-2 diabetics without DN, and 82 type-2 diabetics with DN confirmed by urine microalbuminous detection. Results: The frequencies of MTHFR gene variant genotypes and alleles in the patients with DN patients were significantly higher than those in the patients without DN and those in healthy controls (P<0.01). Conclusion: MTHFR gene C677T mutation is probably one of the genetic risk factors for diabetic nephropathy in Chinese Hans.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
2001年第4期276-278,共3页
Chinese Journal of Medical Genetics
基金
全军医学中青年人才基金&&
