摘要
目的 :测定血清、尿样和胆汁中呋苄西林和其代谢物的含量。方法 :高效液相色谱法 ,AlltimaC18(4 6mm× 15 0mm ,5 μm) ;流动相为 pH 3 0的 0 0 6mol·L-1磷酸二氢钠 (磷酸调 pH 3 0 ,含磷酸四丁基铵离子对试剂 ) -甲醇 -乙腈(5 8∶2 5∶17) ;检测波长 :2 70nm。结果 :血清样品经过甲醇沉淀离心 ,尿样和胆汁样品经过离心、稀释、滤过后即可在HPLC色谱柱上获得良好的分离并同时测定其浓度。对呋苄西林和其代谢物 ,其浓度分别在 0 5～ 12 8μg·mL-1的范围内 ,与峰面积呈线性关系 (r>0 999)。平均回收率在 96 %以上 ,最低检测浓度为 0 0 5或 0 1μg·mL-1,日内、日间精密度RSD小于 7%。结论 :方法简便、快速、准确 ,可以作为呋苄西林和其代谢物在人和动物体内的测定方法。
Objective:To determination the concentrations of furbenicillin and its metabolites in biological samples.Methods:a HPLC method was used,chromatographic conditions included Alltima C 18 column and pH 3 0,0 06 mol·L -1 NaH 2PO 4 (adjust pH to 3 0 with H 3PO 4,containing acid ion pair regent) -methanol-acetonitrile(58∶25∶17)as mobile phase,UV 270 nm as the detection wavelength.Results:Serum samples were precipitated with methanol and centrifuged while urine and bile samples were centrifuged and diluted,furbenicillin and its metabolites could be assayed simultaneously and good separation could be achieved.Calibration curves for furbenicillin,metabolites 1(M1) and metabolites 2(M2) were linear over the range from 0 5 to 128 μg·mL -1 with r >0\^999.The detection limits were 0\^1 μg·mL -1 for furbenicillin,0 05 μg·mL -1 for M1 and M2.The recovery was greater than 96% and overall RSD of within-day and day to day were less than 7\^0%.Conclusion:This method was simple,quick,sensitive and accurate,and had been successfully used for the determination of furbenicillin and its metabolites in serum,urine and bile of animals and human.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第4期225-227,共3页
Chinese Journal of Pharmaceutical Analysis
