摘要
电镜观察粘虫(Leucania separala)核型多角体病毒的不同株系内蒙株(简称N株)和阜阳株(简称F株)的多角体、病毒粒子及核衣壳在形态大小方面都没有很大差别,DNA分子大小也相近。但在SDS-PAGE多肽图谱及DNA酶切图谱上则表现出一定的差异。经SDS-PAGE所得病毒粒子多肽图谱N株有17条多肽,F株有19条多肽。两株病毒DNA经BamHI酶切N株获得7条带,F株则有8条带,说明DNA酶切位点不尽相同。据此,生化分析能更快速、准确地进行株系分类。
Two strains, N and F, of nuclear polyhedrosis virus were isolated from infected Leucania separata in different places. The morphology of these two rains is identical, but their biochemical properties are different. The SDS-PAGE map showed 17 polypepties bands for N strain and 19 bands for F strain. The BamHI restriction map showed 7 fragments for DNA of N strain and 8 fragments for DNA of F train.
关键词
多角体病毒
核型多角体
理化性质
Leucania separata NPV
Virus particle
SDS-PAGE map of Virus polypepties
Restriction map of virus DNA
