摘要
目的 测定女贞子中红景天苷的含量。方法 用反相高效液相色谱法 ,流动相为甲醇 水 (7.5∶92 .5 ) ,紫外检测波长2 78nm ,流速 1.6mL·min-1。结果 红景天苷对照品在 7～ 45 6mg·L-1浓度内线性关系良好 ,r=0 .9997。平均加样回收率97.2 % ,RSD为 3.4%。结论 该法简便 ,分离完全 ,结果可靠 ,适用于女贞子中红景天苷的测定。
OBJECTIVE: To determine the content of salidroside in Nuzhenzi. METHODS: RP-HPLC was applied. The mobile phase was consisted of methanol: water(7.5: 92.5). The detection wavelength was at 278 nm and the flow rate was 1.6 mL·min-1. RESULTS: The linearity was obtained over the range of 7 ∼ 456 mg·L-1 (r = 0. 999 7). The average recovery was 97.2% with relative standard deviation of 3.4%. CONCLUSION: The method was simple, rapid and reliable. It is suitable for the determination of salidroside concentration.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第11期760-761,共2页
Chinese Pharmaceutical Journal
