摘要
褐飞虱 5龄若虫在不同除草剂不同剂量处理的水稻上短期取食 (取食 1h)和长期取食 (3龄若虫取食至 5龄 ,约 7d) ,然后测定其体内代谢酶、保护酶的变化。褐飞虱取食除草剂处理的水稻后其体内羧酸酯酶活性不论是短期取食还是长期取食均极显著高于对照 (未施用除草剂 ) ;乙酰胆碱酯酶活性明显高于对照 ;稻乐思、苯达松低剂量、丁草胺高剂量处理短期取食后超氧化物歧化酶活性显著低于对照 ,所有处理长期取食明显高于对照 ;过氧化物酶活性除了稻乐思 30 0 m L/ hm2和苯达松 15 0 0 m L/ hm2处理短期取食低于对照外 (但没有达显著差异 ) ,也不同程度地高于对照。褐飞虱在除草剂处理的水稻上蜜露排泄量显著增加 ,水稻受害程度与对照相比极显著加重。除草剂处理后的水稻游离氨基酸的含量增加而蔗糖含量下降 。
Metabolization enzymes activity, including carboxylesterase(CarE) and acetytcholinesterrase(AchE) and protective enzymes activity including superoxide dismatese (SOD) and peroxidase (POD) after 5th instar nymph short term feeding(1 d) and long term feeding (third to fifth instar, 7 days) of brown planthopper (BPH), Nilaparvata lugens on the rice plant treated with different herbicides and rates were tested. The results indicated that activities of CarE and AchE of BPH feeding on herbicide treated rice were significantly higher than those of control. SOD activities of BPH feeding for 1 h and 7 days were higher than those of control under metolachlor (300 mL/hm 2), bentazone low dosage and butachlor high dosage treatments. POD activities of other treatments were also higher than those of control, except short term feeding of BPH under metolachlor (300 mL/hm 2) and bentazone (1500 mL/hm 2) treatments. Honeydew excretion amount of BPH feeding on the rice with herbicide treatments significantly increased. Damage scale of rice plant of all treatments was higher than that of control under the same BPH density and same environmental condition. One of causes of benefit to BPH feeding was increased in free amino acids and decreased in sucrose or ratio of C/N. It is suggested that there was two way effect of herbicide application on both rice and BPH.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2001年第4期303-308,共6页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目 ( 39770 12 8)
国际水稻研究所资助项目
