摘要
目的 探讨p5 3、血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)的表达和肿瘤内微血管密度 (intratumoralmicrovesseldensity ,IMVD)测定在人脑星形细胞瘤中的意义。方法 采用免疫组织化学方法 ,检测 6 0例脑星形细胞瘤患者手术切除标本p5 3、VEGF的表达 ,并用抗FⅧ因子抗体标记瘤组织血管内皮细胞 ,计算IMVD。结果 p5 3、VEGF总阳性表达率分别为 45 .0 % (2 7/ 6 0 )和6 5 .0 % (39/ 6 0 ) ,p5 3、VEGF的表达和IMVD均与脑星形细胞瘤组织病理分级呈正相关 (P <0 .0 5 )。p5 3和VEGF阳性表达符合率为 70 .0 % (4 2 / 6 0 ) ,两者的表达有显著性相关 (P <0 .0 5 )。p5 3阳性或VEGF阳性的瘤组织IMVD分别为 75 4± 2 0 0和 74 6± 2 2 0 ,而相应的阴性组分别为 6 2 4± 2 3 9和 5 9 4±2 5 2 ,两者差异有显著性 (P <0 0 5 )。p5 3、VEGF表达均为阳性者 ,IMVD为 75 9± 2 0 3 ;p5 3、VEGF表达均为阴性者 ,IMVD为 5 8 9± 2 2 4;两者差异有显著性 (P <0 0 5 )。结论 (1)p5 3、VEGF的表达以及IMVD的测定可作为判断脑星形细胞瘤恶性潜能的重要生物学指标。 (2 )p5 3、VEGF的表达对肿瘤血管形成可能起重要作用 ,联合检测p5 3、VEGF的表达对了解肿瘤血管形成的机制有一定意义。
Objective This investigation was done to show the pathological importance of p53 protein detection, vascular endothelial growth factor expression (VEGF) and intratumoral microvessel density (IMVD) in astrocytoma. Methods The surgical specimens from 60 brain astrocytoma patients were stained immunohistochemically for p53 and IMVD expressions. IMVD was calculated by labeling the endothelial cells of the blood vessels within the tumor. Results p53, VEGF expression were closely correlated with histopathological grade of astrocytoma. Positive p53 protein accumulation and VEGF expression were found in 45.0% (27/60) and 65.0% (39/60) of tumors. The conformation rate of p53 and VEGF was 70.0% (42/60). IMVD was significantly higher in the p53 positive or VEGF positive tumors than in the negative ones ( P <0.05). Comparing the IMVD in p53+/VEGF+group, p53-/ VEGF+group and p53+/VEGF group, p53-/VEGF-group, its difference was also highly significant ( P < 0 05). Conclusion (1) p53 protein detection, VEGF expression and IMVD can be considered as a biological indicator of malignant potential in brain astrocytoma patients and (2) p53 and VEGF expressions, both contributing to the tumor neovascularization, may be helpful in the understanding of intra tumoral angiogenetic mechanism in the future.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2001年第4期326-329,共4页
Chinese Journal of Oncology
基金
广东省科委重点攻关基金资助项目(1998530158)
广东省"五个一"科教兴医工程重点资助项目(1996343470)
