摘要
目的检测汉滩病毒陈株感染HUVEC细胞系ECV304中炎症介质的表达。方法用汉滩病毒陈株感染HUVEC细胞系ECV304,取感染后不同时间的细胞提取细胞总RNA,用半定量RT-PCR检测感染细胞中ICAM-1、eNOS和IL-6基因的表达,并将扩增的ICAM-1和eNOS基因克隆,测定核苷酸序列。结果IACM-1的表达产物在病毒感染后较未感染细胞有明显升高,序列分析结果表明扩增的基因为特异性扩增产物;用扩增eNOS的引物从病毒感染细胞可扩增出表达产物,从未感染细胞不能扩增出任何产物,但序列分析结果表明扩增的基因与一新基因高度同源。结论汉滩病毒陈株感染ECV304细胞可上调炎症某些介质的表达。
Aim To detect the expression of inflamma tory mediator in HUVEC cell line ECV304infected by Chen strain of Hantaan virus.Methods Total RNA was extracted from ECV304c ells infected by Chen strain of Hantaan virus with differe nt intervals and subjected to revers e transcription.Semi -quantitative RT -PCR was used to detect the mR-NA of ICAM -1、eNOS and IL -6.The PCR products ampli fied with I-CAM -1and eNOS primers were cloned an d sequenced.Results I-CAM -1mRNA was elevated after infect ion with Hantaan virus,and the amplified products were confirmed b y sequence.IL -6mRNA was un-changed after infection.The PCR pro ducts amplified with eNOS primers were detectable after infection,bu t were undectable in mock cells.Se-quence analysis showed that the ampl ified gene was highly homologous with a newly accessed cDNA.Conclusion the expression of some inflam-matory mediators can be upregulated by the infection of Hantaan virus.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第5期417-418,共2页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助
No.39970702
