摘要
整合了含乙型肝炎病毒表面抗原(HBsAg)基因和dhfr基因的CHO-dhfr^-细胞,其染色体的畸变率和畸变类型都比亲代CHO-dhfr^-细胞高。但转化前后两系细胞的重要特性都未发生变异,即两者的染色体总数无差别,都是20条。两系细胞株接种裸鼠,均未发现有致瘤性。
The frequencies and types of chromosome aberrations induced by integration of plasmid containing of hepatitis B surface antigen and dihydrogen folic acid reductase (dhfr) into CHO-dhfr- (DXB-11) cells were found to be clearly higher when compared with that of the original CHO-dhfr-cells without integrated DNA. There is no change in the number of chromosomes, both cell lines having 20 chromosomes. Both cell lines were non-oncogenic in nude mice.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第4期323-326,共4页
Chinese Journal of Virology
关键词
HBSAG
重组
DNA
染色体畸变
Hepatitis B surface antigen Dihydrogen folic acid reductase Chinese hamster ovary cell Chromosome aberration Carcino-genicity
