摘要
目的:探讨重组人血小板生成素对失血性血小板减少小鼠的影响。方法:以Babl/c纯系小鼠为实验对象,分成实验组及阴性对照组。实验前采尾静脉血,了解两组小鼠血小板正常值,然后通过腹腔连续7天注射重组的人血小板生成素,于第7天及第14天采眶静脉血,并制成失血性血小板减少的动物模型;观察重组人血小板生成素在小鼠体内的促血小板生成的生物学活性及其对小鼠的生存情况影响。结果:在实验第7天,实验组小鼠外周血血小板数明显高于同期阴性对照组(P<0.05);而且随实验时间的延长,实验组血小板数有升高趋势。另外,在第7天眶静脉采血后,两组失血性血小板减少小鼠陆续出现死亡,但实验组小鼠死亡率明显低于阴性对照组(P<0.05)。结论:血小板生成素体促进小鼠外周血血小板的产生,该生物学活性在一定程度上降低了血小板减少小鼠的死亡率。
Objective: To investigate effect of recombinant human thrombopoietin on exsanguine thrombocytopenia mice. Methods: Normal peripheral platelet counts were performde on sample obtained from the tail vein of purebred Babl/c mice including to experimental and control groups before experiment. rhTPO were injected into the mice respectively in 7 days by intraperitoneal injection once for a day. On the seventh and the fourteenth day, the mice were phlebltomized from the supra-obitalis vein in order to make exsanguine thrombocytopenia animal model. At the same time, we observed the biological activity of recombinant human thrombopoietin in vivo and the condition of the mice's survival. Results: On the seventh day and the fourteenth day, the platelet counts of mice treated by rhTPO were higher than by PBS(P < 0.05). Moreover the platelet counts of mice in experimental group of rhTPO showed increasing direction following experimental days. In addition, death happened in two groups after those mice were phlebotomized from the supra-obitalis vein, but the death rate of negative control group was evidently higher than xeperimental group ( P < 0. 05 ). Conclusion: rhTPO had obvious biological activity in increasing platelet production, which resulted in the drop of thrombocytopenia mice s death rate in the experimental groups.
出处
《血栓与止血学》
2001年第3期101-103,共3页
Chinese Journal of Thrombosis and Hemostasis
