摘要
目的 :探讨抗细胞间粘附分子 - 1(ICAM- 1)单克隆抗体 (单抗 )对过敏性小鼠哮喘模型肺组织 T淋巴细胞增殖反应和活化状态的影响。方法 :应用鸡卵清蛋白致敏和刺激小鼠以复制过敏性哮喘模型 ,收集肺组织 T细胞并以流式细胞仪检测其增殖反应 ,以及活化后产生白细胞介素 - 5 (IL- 5 )的能力。结果 :哮喘小鼠肺组织 T细胞的增殖反应明显增强 ,肺组织局部所产生的 IL- 5的水平也显著增高。经抗 ICAM- 1单抗处理后 ,T细胞增殖能力显著降低 ;同时 ,抗 ICAM- 1单抗还可以抑制 IL- 5的产生。结论 :抗 ICAM- 1单抗能够抑制肺组织局部 T细胞的增殖以及 IL- 5的产生。
Objective:The aim of the present study was to explore the effects of anti-intracellular adhesion molecule-1(ICAM-1) monoclonal antibody (mAb) on proliferation of T lymphocytes and IL-5 production in sensitized mice lungs, and to futher elucidate the role of ICAM-1 in the pathogenesis of bronchial asthma. Methods: Female BALB/c mice were sensitized and challenged with ovalbumin to prepare animal asthma model. Flow cytometry was used to determine proliferation response of lung T cells, and interleukin-5 concentration in homogenized supernatant of lung tissue were determined by ELISA. Effect of anti-ICAM-1 mAb on proliferation of T lymphocytes and IL-5 production were observed. Results: In sensitized mice challengend with ovalbumin 20 minutes once a day for 6 days, the number of proliferating T cells in lung tissue was much higher that that in control mice. In mice treated with intravenous injection of anti-ICAM-1 mAb before each challenge, proliferating T cells dereased by 54%.the results also showed that anti-ICAM-1 mAb prevented antigen-induced production of interleukin-5 in lung tissue.Conclusions:Anti-ICAM-1 mAb is able to inhibit antigen-induced proliferation of pulmanary T cells as well as productions of interleukin-5. These data suggested that the blockade of antigen-presenting cells'functions in airways could be of value in treatment of human asthma.
出处
《广西医科大学学报》
CAS
2001年第3期346-348,共3页
Journal of Guangxi Medical University
