摘要
用番鸭呼肠孤病毒B3分离株接种番鸭胚、半番鸭胚和鸡胚 ,导致接种胚死亡 ,并产生基本一致的病理变化 :死亡胚周身出血 ,肝、脾有灰白色坏死点 ;感染番鸭肝细胞出现颗粒变性 ,随后崩解。该病毒具有经胚蛋、滴鼻、饮水、肌肉和爪垫注射与同居感染的能力 ,潜伏期 3～ 11天 ,不同途径接种 1日龄敏感番鸭死亡率达 10 0 % ;接种 1日龄番鸭、半番鸭和雏鸡爪垫可引起注射部位炎性反应 ,爪垫和肌肉注射 1日龄半番鸭和雏鸡不引起死亡。感染番鸭主要可见肝、脾、心肌、肾、法氏囊、腺胃、肠粘膜下层等组织局灶性坏死 ,其中以肝、脾尤为显著 ,肝组织和脾白髓结构遭到严重破环 ;肝、脑血管周围和肾间质、肺间质、心肌间有淋巴样细胞或 /和吞噬细胞聚集 ,法氏囊淋巴细胞变性和坏死。电镜观察感染胚肝细胞和脾淋巴细胞胞浆内有大量病毒样颗粒及近核包涵体 ,感染细胞多核化、空泡化及颗粒化 ;可见含有病毒样颗粒的凋亡细胞 ;
The general haemorrahage and gray_white necrotic foci were observed on the surface of liver and spleen of the dead embryos of muscovy ducks,hybrid(muscovy)ducks or chickens inoculated with the muscovy duck reovirus isolate B3.The infected liver cells of muscovy duck showed the granular degeneration,and then lysis,The experiments confirmed that the isolate B3 could be transmitted through infected eggs or by nasal,oral,intramuscular(i.m.),pad injection and co_breeding.The incubation period of one_day_old muscovy ducks inoculated with isolate B3 was from 3 to 11 days,the rate of mortality was up to 100%,but some resistance to the isolate B3 were found in the one_day_old hybrid(Muscovy)ducks or chickens.There were inflammatory responses caused by the pad inoculation to the muscovy ducks,hybrid(muscovy)ducks or chickens of one_day_old,though the apparent clinical signs didn′t happen by i.m.injection on hybrid(Muscovy)ducks or chickens.Macroscopically,fibrinous pericarditis,gray_white necrotic foci,enlarged friable liver,and marbled spleen were seen in dead muscovy ducks infected with the isolate B3.Microscopical observation found that there were focal necroses in liver,spleen,cardiac muscle(heart),kidney,bursa,proventriculus and intestinal submucosal coat,and they were especially appatent in liver and spleen,and the tissue structure of splenic white pulp and liver was destroyed.Lymphocytic and phagocytic infiltrations were consistently found in the perivascular of the liver and brain,and in the interstice of cardiac muscle,kidney and lung.The lymphocytic degenerations and necroses were also seen in the bursa of Fabricius.EM examinations revealed that there were virus_like particles and inclusion bodies near the nucleus;multinuclear,vacuollization and granular degeneration were found in the liver cells and the lymphocytes of spleen of infected embryos.The isolate B3 also resulted in apoptosis,virus_like particles and apoptic bodies in the cytoplasm of phagocytes.The findings were firstly reported so far in china.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第6期422-425,共4页
Chinese Journal of Preventive Veterinary Medicine
关键词
番鸭
呼肠孤病毒
肝白点病
致病性
Muscovy duck
Reovirus
Liver white_spots disease
Pathogenicity
