摘要
建立了检测呼吸道合胞病毒(RSV)和副流感病毒(PFV)血清特异性IgM和IgA抗体的间接ELISA方法。在方法统一的基础上比较了检测IgG、IgM和IgA抗体的结果,证明检测血清IgM和IgA可以作为RSV和PFV感染的早期诊断指标。检测了120份临床急性下呼吸道感染患儿的血清,RSV-IgM检出率为33.3％,RSV-IgA为36.7％;PFV-IgM为27.5％,PFV-IgA为31.6％。提出了对RSV和PFV感染以检测特异性IgA替代IgM或两者互补的设想。
Indirect ELISA methods were set up for the detection of specific serum IgM and IgA antibodies for respiratory syncytiai virus(RSV) andparainfluenza virus (PFV) infection in lower respiratory tract. We madea comparison of detection of IgM, IgA, IgG classes immunoglobulins in children with RSV or PFV infection, which suggests both IgM and IgA antibody can be interpreted as a sign of early diagnosis. Of the 120 sera from children with acute lower respiratory infections,RSV-IgM 40 (33. 3%), RSV-IgA 44(36.7%) and PFV-IgM 33 (27.5%) , PFV-IgA 38 (31.6%) were found positive. It was suggested that detection of IgA and IgM may complement each other for the diagnosis of RSV, PFV infection of lower respiratory tract.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第4期383-387,共5页
Chinese Journal of Virology
关键词
呼吸道
合胞病毒
副流感病毒
Acute lower respiratory infection Respiratory syncytial virus Parainfluenza virus IgM IgA ELISA
