摘要
本文对采自不同地区的小菜蛾种群幼虫不同亚细胞层羧酸酯酶 (Car E)的活性分布及性质进行了研究。结果表明 ,敏感品系和抗性种群 Car E活性亚细胞分布没有明显差异。不同种群各亚细胞层 Car E活性以线粒体层分布最高 ,占 34%～ 52 % ;微粒体层最低 ,占 7%～ 16 %。抗性种群线粒体层 Car E活性明显高于敏感品系。各种群以上清液层 Car E与 α- NA的亲和力最高 ,且各种群间没有明显差异。云南、北京、武汉、宣化种群(F0 )线粒体层 Car E与 α- NA的亲和力最低。测定的六个种群线粒体层 Car E与 α- NA的亲和力大小依次为 :AVMs抗性品系 (F16) >云南种群 >武汉种群 >台湾敏感品系 >宣化种群 (F0 ) >
Subcellular distribution of carboxylesterase (CarE) activities and the properties of CarE were investigated in diamondback moth (DBM), -Plutella xylostella,- collected from Xuanhua, Hebei Province, Wuhan, Hubei Province, Kunming, Yunnan Province, Beijing city, AVMs-selected resistance strain and susceptible strain in 1997. There was not significant difference of subcellular distribution of CarE activity among the susceptible strain and the resistant populations or strain. The major portion (34%~52%) of CarE activity was associated with mitochondria fraction and a little (7%~16%) with microsome fraction among four fractions in all populations tested. The CarE activity in mitochondria fraction of resistant populations was higher than that of susceptible strain. The affinity of CarE in supernatant fraction toward α-naphthyl acetate (α-NA) was highest among four fractions and no difference in all populations tested. The affinity of CarE in mitochondria fraction toward α-NA was lowest among four fractions in Yunnan, Beijing, Wuhan and Xuanhua populations. The affinity of CarE in mitochondria fraction toward α-NA was AVMs-selected strain (F 16 ), Yunnan, Wuhan, Taiwan susceptible strain, Xuanhua, Beijing population in order of decrease.
出处
《农药学学报》
CAS
CSCD
1999年第2期47-53,共7页
Chinese Journal of Pesticide Science
