摘要
目的 研究端粒酶逆转录酶基因hTERT在膀胱移行细胞癌 (TCC)中的表达 ,探讨其在TCC发生、发展及预后复发中的意义 .方法 采用兔抗人hTERT多克隆抗体及S P免疫组化法检测在 5 6例TCC及 10例正常膀胱组织中的表达 .结果 hTERT在TCC中阳性表达率为 6 4.3% ,与在正常膀胱组织表达率 0 %相比 ,有显著性差异 (P <0 .0 5 ) .hTERT在Ⅰ ,Ⅱ和Ⅲ级TCC中的表达率分别为 43.8% (7/16 ) ,5 7.7% (15 / 2 6 ) ,10 0 % (14/ 14) .Ⅰ与Ⅲ级、Ⅱ与Ⅲ级、Ⅰ+Ⅱ与Ⅲ级比较差异显著 (P <0 .0 5 ) .hTERT在浅表性及浸润性TCC中的表达率为 5 3.1% (17/ 32 ) ,79.2 % (19/ 2 4) ,两者差异显著 (P <0 .0 5 ) .hTERT阳性表达率与患者复发有显著相关性 (P <0 .0 5 ) .结论 hTERT表达异常可能与膀胱肿瘤的发生、发展有关 .
AIM To investigate the expression of human telomerase reverse transcriptase gene (hTERT) and to evaluate its clinical significance in the occurrence , development and recurrence of bladder transitional cell carcinoma (TCC) patients. METHODS Using S P immunohistochemical method, we studied the expression of hTERT gene protein in 56 cases of paraffin embedded TCC tissues and in 10 cases of normal bladder tissues. Their clinical significance was analysed. RESULTS The positive expression rates of hTERT gene were 64.3% in patients with TCC and 0% in the normal bladder tissues respectively ( P <0.05). hTERT protein positive staining was in the cytonucleus of TCC. Significant difference of hTERT gene expression was observed in TCC of pathological grades Ⅰ, Ⅱ and Ⅲ, its positive expression rates were 43.8%(7/16),57.7% (15/26), 100% (14/14) respectively. Statistical significance was found in Ⅰ and Ⅲ, Ⅱ and Ⅲ, Ⅰ+Ⅱ and Ⅲ respectively ( P <0.05). hTERT gene expression was related to the clinical stages and its positive expression rates among superficial and invasive tumor were 53.1% (17/32) and 79.2% (19/24) respectively ( P <0.05). The expression of hTERT was significantly positively related to the recurrence of the patients ( P <0.05). CONCLUSION The results suggest that hTERT gene expression is over expressed in TCC and the aberrant expression of hTERT gene is closely related to the tumorgenesis and progression of TCC. The highly expression of hTERT gene might be regarded as a marker for the recurrence of TCC.
出处
《第四军医大学学报》
北大核心
2001年第15期1412-1415,共4页
Journal of the Fourth Military Medical University
关键词
膀胱肿瘤
端粒酶
基因表达
免疫组织化学
bladder neoplasms
telomerase
gene expression
immunohisto chemistry
