摘要
目的 :探讨早期切创创壁肌肉损伤病变及发展过程 ,为法医学鉴定早期损伤时间提供病理学基础。方法 :用手术刀切开Wistar大鼠大腿肌肉 ,分别于即刻、1分钟、5分钟、15分钟、30分钟、45分钟及 6 0分钟与死后不同时间作对照 ,在电镜下观察其形态改变。结果 :即刻时透射电镜观察肌肉未见异常 ;损伤 1分钟、5分钟和 15分钟时透射电镜观察肌肉 ,线粒体水肿空泡化从无到有 ,肌原纤维小灶性坏死随时间推移逐渐加重 ,肌原纤维间隙增宽越来越明显。损伤 30分钟、45分钟和 6 0分钟时 ,线粒体水肿空泡化更加严重 ,嵴消失 ,肌原纤维缩短呈波浪状改变 ,坏死、断裂并浓缩成块状 ,肌丝排列紊乱。核异质多 ,不规则 ,核周出现空泡 ,染色质均质化 ,肌浆见大量不等空泡 ,肌纤维大量坏死。死后损伤超生反应及波浪状改变以 15分钟时段最为明显。结论 :提示早期创壁肌肉损伤后不同时间各有其形态学特征 ,这为早期损伤时间推断的法医学鉴定提供了病理形态基础。
Objective:To explore the pathogenesis of early incised wound surface injuries.Methods:Animal model of early incised wound surface was established by surgical incisions of the rat muscle on an instant basis and l min,5min、15min、30min、45min、and 60min basis respectively. Structural changes were observed under electron microscope and compared with postmortem muscle injuries of different time.Results:No significant abnormality was observed on an instant injury.Vacuole and edema of mitochondria become increasingly serious. Small and local necrosis of myofibril fibers become more obvious and interstice of myofibril fibers become widened on lmin、5min、15min、inujries. On 30min、45min、and 60min injuries,increasing severity was observed. Vacuole and edema of mitochondria become very serious loss of crista. Contracting and waving pattern of some myofibril fibers also become very serious and local necrosis of myofibril fibers more obvious. Disorder arrangement of myofilament was found. Some myofibril fibers were broken and contracted into blocks.Irregular intranuclear inclusions perinuclear vacuole,chromatin homogenization large amount of unequal vacuole were seen in sarcoplasm. Major necrosis were seen in myofilament fibers.Supravital reaction and waving pattern were most obious in postmortem injury 15min.Conclusion:The experiment provids the pathological basis for the forensic investigation of early injury time deduction by observing structural changes of early incised muscle injury of different times.
出处
《温州医学院学报》
CAS
2001年第3期142-144,共3页
Journal of Wenzhou Medical College
