摘要
目的 :研究慢性上颌窦炎患者耐甲氧西林金黄色葡萄球菌 (MRSA)的分布和对常见抗菌药物的耐药情况。方法 :采用多重聚合酶链反应 (PCR)同时检测金葡菌的耐药基因 mec A及辅助基因 fem A ;药敏试验采用纸片扩散法。结果 :fem A基因为金葡菌的特有基因 ,mec A基因在 MRSA中检出率为 96 .2 % (5 1/ 5 3)。 MRSA除对万古霉素敏感外 ,对其它抗菌药物都有耐药。结论 :多重 PCR能快速而准确地鉴定 MRSA;MRSA对多种抗菌药物有耐药性。
Objective:To investigate the detection method of methicillin resistant staphylococcus aureus (MRSA) in chronic maxillary sinusitis and the situation of drug resistance to some common antibiotics.Method:Using multiplex polymerase chain reaction technique to detect the drug resistant gene (mecA)of MRSA and specific gene(femA) of staphylococcus aureus at the same time. Drug resistant tests were performed with disk diffusion (K B) method. Result:femA is a specific gene of staphylococcus aureus, the detective ratio of mecA in MRSA is 96.2 % (51/53). Vancomycin is the only antibiotic which is sensitive to MRSA, while others are not. Conclusion:MRSA can be rapidly and specifically identified with multiplex PCR.There are drug resistance to many antibiotic drugs for MRSA.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2001年第8期341-343,共3页
Journal of Clinical Otorhinolaryngology
