摘要
目的 :研究健康受试者口服盐酸班布特罗后血浆中特布它林浓度 LC-MS测定方法。方法 :血浆中特布它林溶剂提取后采用选择性离子检测方法测定其血药浓度 ,1 0 % Na2 S2 O5溶液作为酶抑制剂。结果 :特布它林的线性范围为 0 .1 ng/ ml～ 2 0 .0 ng/ ml,日内和日间精密度均小于 1 5 .0 % ,方法的回收率为 79%～ 90 %。结论 :该测定方法学考察均符合血浆样品的要求 。
AIM A highly sensitive and specific method for the determination of terbutaline in human plasma by LC MS was developed after administration of its prodrug Bambuterol. METHODS\ Terbutaline was extracted from human plasma and analyzed by LC MS via an electrospray ionization interface. Selected ion monitoring was used to detect terbutaline and its internal standard. To inhibit the esterase catalyzed hydrolysis of bambuterol to terbutaline ,10% Na 2S 2O 5 solution was added to the plasma sampling tubes. RESULTS The intra day variation on Spiked samples ranged from 3.7% to 10.5% and The inter day variation on Spiked samples ranged from 9.46% to 11.47%. The lowest limit of quantitation was 0.1 ng/ml when 1.0ml plasma was used.The absolute recovery was in the range 79% 91%. CONLUSION The method is specific and sensitive enough to obtain pharmacokinetic data from clinical trials.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2001年第5期359-362,共4页
Journal of China Pharmaceutical University
