摘要
于梨状肌下缘１０ｍｍ处切除成年Ｗｉｓｔａｒ大白鼠右侧的坐骨神经５ｍｍ，移植入８ｍｍ长、经２×１０￣６ｒａｄ￣（６０）Ｃｏ辐射的成年日本大耳兔胫神经。异种神经移植术后８、１２、１６、２０、３２周，取出鼠手术侧的爪皮入１０％福尔马林固定、冰冻切片，按Ｇｒｏｓｓ－Ｓｃｈｕｌｔｚｅ改良的Ｂｉｅｌｓｃｈｏｗｓｋｙ法染色显示皮肤感觉神经末稍。术后８周的爪皮皮下组织出现少量再生神经束，１２周后的各存活期真皮层内部分再生神经纤维可能与Ｍｅｉｓｓｎｅｒ小体和Ｍｅｒｋｅｌ触盘的形成有关。３２周的部分再生神经纤维穿行于表皮细胞之间形成游离感觉神经末稍。随着存活期的延长，皮肤感觉神经末稍的数量和种类逐渐增多。￣（６０）Ｃｏ辐射的异种神经移植后，宿主的再生神经纤维能向远段生长重新支配靶器官。
Right sciatic nerve of the adult wistar rat was exposed and a 5 mm segment was cut fromthe point 10 mm distal to the lower margin of pirform muscle. Nerve segment of 8 mm inlength excisae from the adult rabbits(Japanese big ear rabbit)and treated by 2×10￣6 rad￣(60)Coirradiated was transplanted into the gap of the severde rat nerve。Four anirnals at each timewere sacrificed after being grafted for 4,8,12,16,20 and 32 weeks。The toe pads of theoperated limb were excised and stained with Bielschowsky modified by Gross-Schultze to re-veal sensory nerve endings of the skin.There were regenerating nerve fascicles in subcuta-neous tissue of the toe pads at 8 weeks post-graft.At 12,16,20 and 32 weeks after trans-plantion,some regenerating nerve fibers in dermis associated with the formation of Meissners corpuscle and Merkel’s disc。Some fibers were distributed among the epidermal cells foum-ing free nerve endign after 32 weeks。As regeneration proceeded,the sensory nerve endingsof skin increased in number and type.The regenerating nerve fibers of the recipient couldpass through the heterograft nerve to the target organ after heterograft nerve irradiated by￣(60)Co.
出处
《江西医学院学报》
1994年第2期5-7,F002,共4页
Acta Academiae Medicinae Jiangxi
基金
国家自然科学基金
关键词
异种移植
胫神经
坐骨神经
神经移植
^60CO辐射
免疫抑制剂
transplantation,heterologous/methods
tibial nerve/transplantation:sciatic nerve
animals,Iaboratory
rabbit
rat
