摘要
目的 应用同种异体兔成骨细胞研究组织工程化人工骨对骨缺损的修复能力。方法 取 4周龄新西兰幼兔的颅骨组织 ,应用胰酶及I型胶原酶消化 ,经分离、培养而获得的成骨细胞种植到可降解聚合物 (PLGA)泡沫材料上 ,体外培养 1周后 ,将两者的复合体移植到兔颅骨缺损区域 ,细胞接种浓度为 4× 1 0 10 个 /L。分别于术后 4、8、1 2周取材 ,进行大体、X线、组织学及免疫组织化学观察。以缺损区单纯植入泡沫材料或单纯种植成骨细胞为对照组。结果 成骨细胞 支架复合体组颅骨缺损修复明显 ,两对照组颅骨缺损无明显修复。术后 1 2周 ,对各组免疫组织化学染色结果进行图像分析 ,其中实验组灰度值为 92 .6,单纯泡沫材料组与单纯种植成骨细胞组灰度值分别为 1 3 1 .7及 1 1 9.3。结论 本实验构建的组织工程化人工骨具有较强的颅骨缺损修复能力 。
Objective To study the capacity of the tissue engineered bone to repair the defect of the calvarial bone using polymer substrates and osteoblasts.Methods Osteoblasts were enzymatically isolated from the calvarial bone of the 4 week old rabbits,and then seeded into foams of PLGA with a cellular density of 4×10 10 /L.After incubation for 1 week in vitro, the osteoblast polymer constructs were implanted into the calvarial bone defects of the rabbits. The implants were then taken out at 4, 8, 12 weeks postoperation separately, and dissected for macroscopic, X ray, histological and immunohistochemical examinations.Results The osteoblast polymer constructs were progressively replaced by more mature bone tissue as compared with two control groups. The result of image analysis for the immunohistochemical examination at 12 weeks was 92.6 for experimental group and 131.7 or 119.3 for polymer only group and osteoblast transplantation group respectively.Conclusion The tissue engineered bone owned excellent bone repairing ability, and the biodegradable foam of PLGA is a good 'matrix' for osteoblasts.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第5期445-447,共3页
Chinese Journal of Experimental Surgery
基金
国家高技术航天领域项目 (86 3 2 7 2 1 5 )
国家自然科学基金青年基金资助项目 (3 990 0 0 3 6 )
