摘要
目的 探讨心脏移植术后单用雷公藤多甙 (TWHF)及与环孢素A(CsA)合用对大鼠心脏移植物生存期的影响。方法 供体为雄性PVG(RT1 C)大鼠 ,受体为雄性Lewis(LEW ;RT1 1)大鼠。采用改良的Ono和Lindsey方法实施腹部异位心脏移植。根据术后处理不同将实验动物分为 4组 :第 1组 (5只 ) :对照组 (非治疗组 ) ;第 2组 (7只 ) :TWHF 60mg·kg-1·d-1;第 3组 (6只 ) :CsA 1 5mg·kg-1·d-1;第 4组 (5只 ) :TWHF 40mg·kg-1·d-1+CsA 1mg·kg-1·d-1。用吐温 80及蒸馏水将TWHF配制成 1 %悬液 ;CsA使用前以注射用水配成 5g/L。术后当天即给药 ,TWHF为灌服 ,CsA为肌肉注射。直至发生排斥反应或最多给药 1 4d。每天通过触摸监测移植心脏的功能。结果 4组移植大鼠心脏平均存活 8.8、1 2 .0、1 7.0、3 4 .6d。结论 心脏移植术后给予雷公藤多甙及CsA联合治疗 。
Objective To evaluate the effect of tripterium wilfordii hook f (TWHF) alone or in combination with cyclosporine A (CsA) on heterotopic rat cardiac allograft survival.Methods Heterotopic abdominal heart transplants were performed in the disparate rat strain combinations of PVG(RT1 C)donor to Lewis(LEW;RT1 1)recipient. The transplanted rats were divided into 4 groups according to different administrations listed below: group 1 (n=5): control (untreated); group 2 (n=7) receiving TWHF 60 mg·kg -1 ·d -1 ; group 3 (n=6): receiving CsA 15 mg·kg -1 ·d -1 ;group 4 (n=5) receiving TWHF 40 mg·kg -1 ·d -1 in combination with CsA 1 mg·kg -1 ·d -1 . TWHF was dissolved in a fresh suspension of polysorbate 80 and distilled water to 1%, while CsA was diluted in water to a concentration of 5 g/L and administered daily via gavage and intramuscular injection for at longest 14 days from the day of transplantation. Graft function was monitored daily by abdominal palpation.Results The mean survival time (MST) of the cardiac grafts in the four groups was 8.8, 12.0, 17.0, and 34.6 d, respectively.Conclusion TWHF has a synergistic effect with CsA in prolonging rat cardiac allograft survival.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第5期407-408,共2页
Chinese Journal of Experimental Surgery
关键词
心脏移植
雷公藤多甙
环孢菌素A
移植物排入
大鼠
Heart transplantation
Tripterium wilfordii hook f
Cyclosporine A
Graft rejection
