摘要
应用乙型肝炎病毒核心抗原基因转化的小鼠L细胞分泌的乙型肝炎病毒e抗原,采用ELISA法与Abbott公司抗-HBe EIA诊断盒平行比较,检测了31份抗-HBe阳性和19份抗-HBe阴性的人血清,结果完全相符。经多次重复试验,本法的OD490nm值的误差不超过8％。OD490nm值与血清稀释度之间呈直线关系。细胞培养液不经纯化即可应用,一般做1:4稀释。细胞分泌的抗原无感染性,价格低廉,不会因结合人血清蛋白而产生非特异性反应。因此比一般诊断盒中所用的人血清HBeAg有很大的优越性。
Hepatitis B e-antigen(HBeAg)secreted by genetically engineered L cell was used as antigen in an ELISA test for the detection of hepatitis B e-antibody (anti-HBe) in human sera. Parallel tests of 31 anti-HBe positive sera and 19 anti-HBe negative sera by ELISA using cell-secreted antigen and Abbott anti-HBe EIA kit showed complete agreement of results. The OD 490nm value of the test are reproducible within 8% and a linear line was obtained by plotting OD 490nm against antibody dilutions. The cell supernatant may be used without purification, usually at 1:4 dilution. This antigen is non-infectious, cheap and free from spurious reactions due to associated human proteins. Thus it appears to be superior to the human serum HBeAg routinely used in diagnostic kits.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第2期159-167,共9页
Chinese Journal of Virology
关键词
乙肝病毒
E抗原
E抗体
Hepatitis B e-antigen secreted by mammalian cells Hepatitis B e-antibody ELISA
