摘要
从鲤鱼卵巢中提取核酸(mRNA)注入不同发育阶段的金鱼胚胎中,观察mRNA对尾鳍发育的影响。观察结果表明:一次注射的或多次注射的,在胚胎早期均能使双尾鳍变成单尾鳍,但多次注射的,出现单尾鳍数要高;大剂量注射,甚至还可以使晚期胚胎(耳石期),也能形成单尾鳍;mRNA注入卵黄囊中所产生的单尾鳍鱼要比注入胚盘中为多;失活的mRNA不能诱导出单尾鳍的形成。 从实验所得的资料表明:鲤鱼卵巢mRNA能够诱导双尾鳍金鱼转变成单尾鳍,但这种外源mRNA的作用性质,是由具有活性的mRNA翻译成蛋白质或者以它本身直接影响或干扰胚胎中某种“调节因子”,致使双尾鳍基因不能表达,从而导致单尾鳍的形成。
Poly (A) mRNA obtained from carp eggs was injected into goldfish embryo at different developmental stages to study the effect of the mRNA on the caudal fin development. The experiment consisted of two parts:I. Microinjecdon of mRNA was made only once. The fertilized but uncleaved eggs were injected with carp egg-mRNA in the blastoderms, while all the other embryos were injected in the yolk sacs. The experimental result demonstrated that (1) the caudal fin transformation from the double to the single occurred in the embryos injected with the mRNA at 1-cell stage and in those injected with the mRNA at gastrula stage, or even at the auditory vesicle forming stage; (2) the embryos introduced the mRNA into the yolk sacs gave rise to more single caudal fin goldfish than those introduced the mRNA into the blastoderm.II. Microinjecdon of mRNA was made several times. Some embryos were successively injected three times, some four times, and some five. It showed that the embryos injected with the mRNA successively formed more larva fish with single caudal fin than those injected with the mRNA only once.The available data show that the carp egg-mRNA did induce the transformation of caudal fin from double to single in the goldfish. The foreign mRNA did not influence the host nucleus, but, by translating into new proteins in the cytoplasm, it interferes in the factor regulated the double formation, resulting in the single caudal fin formation.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1989年第6期508-513,共6页
Oceanologia Et Limnologia Sinica
