摘要
本文以花背蟾蜍的胚胎(19～25期)为材料,采用放射自显影技术,以~3H—氨基葡糖标记氨基多糖,并用链霉菌透明质酸酶和睾丸透明质酸酶处理作对照,在光镜水平上研究了透明质酸在角膜早期形态发生中合成变化。结果表明:透时质酸的产生及消除与角膜的形成及分化有相应的关系,在内角膜基质原基开始形成的第20期,即间充质细胞向预定角膜上皮与晶状体之间迁移之际,透明质酸的合成量达到最高水平,此后透明质酸的合成量逐渐减少到最低水平,这相应于角膜上皮开始变薄、透明及分化。本文就透明质酸在角膜早期形态发生中的作用机制进行了初步讨论。
We use ~3H-glycosamine labelling glycosaminoglycans(GAGs) accompaniedby Streptomyces hyaluronidase and testicular hyaluronidase digestion toexplore the changes of hyaluronic acid synthesis during early cornealmorphogenesis in Bufo raddei Strauch by light microscopic autoradiography.The results indicate that the generation and removal of hyaluronic acidrelate to the formation and differentiation of cornea. At stage 20 (gillcirculation stage), when the inner cornea begins to form, i. e., themesenchymal cells invade into the interspace between the presumptivecorneal epithelium and presumptive lens. The amount of hyaluronic acidsecreted by the inner and outer cornea reaches the highest level,hereafter,falls gradually to the lowest.This corresponds to the thinning, transparencyand differentiation of cornea The role of hyaluronic acid in the earlycorneal morphogenesis is discussed.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
1991年第1期75-81,共7页
Journal of Lanzhou University(Natural Sciences)
基金
国家自然科学基金
关键词
花背蟾蜍
角膜
形态发生
透明质酸
Bufo raddei
cornea
morphogenesis
hyaluronic acid
autoradiography
