摘要
将5株甲型肝炎病毒(HAV)分别感染人胚肺二倍体细胞,在各株病毒的细胞培养液中,用cDAN—RNA杂交法可测到HAV RNA,但用ELISA法不能测到病毒抗原,培养液中HAV的TCID50/ml略低于细胞内的病毒滴度。PEG免疫电镜观察到,培养液内的病毒几乎全部是实心颗粒。实验表明,在不同株HAV的培养液内均可测出病毒及病毒RNA,但各株测出的时间不同,讨论了HAV的释放机制。
HAV RNA could be detected by cDNA-RNA hybridization method in the HAV infected cell cultural medium. But HAAg could not be detected by ELISA. The infectivity of HAV in the cultural medium was very high, it's TCID50/ml was only a little lower than that of HAV in the infected cells. It was observed that HAV particles in the cultural medium were nearly all full particles ( mature virion ) by PEG IEM method. The time of HAV appearance fn the cultural medium was different among different HAV strains. A discussion was made about the mechanism of HAV release from the infected cells.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第1期19-23,共5页
Chinese Journal of Virology
关键词
甲肝病毒
二倍体细胞
分子杂交
Hepatitis A Virus 2BS cell Molecular hybridization Secretion
