摘要
目的 :研究神经肽 Y(Neuropeptide Y ,N PY)对心肌缺血再灌注 (m yocardial ischemia reperfusion,MIR)损伤的影响及蝙蝠葛酚性碱 (phenolic alkaloids of m enispermun dauricum ,PA MD)的保护作用。方法 :将实验兔 32只随机分为 4组 :1假手术组 ;2 MIR组 ;3MIR加 NPY组 ;4MIR加 NPY加 PAMD组。观察缺血前 ,缺血 30 min,再灌 10、30 min,1、2、3、4h血液一氧化氮 (NO)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)的含量变化 ,缺血后 10 min给予 N PY静注对上述指标的影响以及缺血前静脉注射钙离子抑制剂 PAMD,NPY对上述指标影响的逆转作用。结果 :1MIR组与假手术组比较 ,NO下降 ,SOD下降 ,MDA上升。2 MIR加 NPY组与 MIR组比较 ,N O、SOD活性均明显下降 (P <0 .0 5 ,<0 .0 1) ,MDA含量明显上升 (P <0 .0 1) ;3NPY对 NO、SOD、MDA的影响可被 PAMD逆转。结论 :NPY通过加重对内皮功能的损伤作用而加重心肌缺血再灌注损伤 ,可能的机制是增加细胞内钙的负荷 ,钙离子通道抑制剂对 NPY的此种效应有逆转作用 ,支持 NPY通过增加细胞内钙负荷 ,使自由基清除能力下降的途径损伤心肌细胞
Objective: To observe the influ en ce of Neuropeptide Y(NPY) and the protective effect of phenolic alkaloids of men ispermum dauricum (PAMD) on myocardial ischemia resperfusion (MIR) injury.Method: Thirty-two rabbits were randomized into four grou ps (n=8 in each): ① Sham operated group, ② MIR group, ③ MIR+NPY group, ④ MIR+NPY+PAMD group. Ntricoxide (NO), Superoxide dismutase (SOD) and malondialde hyde were measured before ligation of the coronary artery, 0.5 h after ische mia, 10 minuts, 0.5, 1, 2, 3, 4 hours after reperfusion.Result: ① Comparing with sham group operation, NO, SOD de creased (P< 0.01) and MDA increased (P< 0.01) after reperfusion in group MIR; ② Comparing with group MIR, No decreased (P< 0.05), SOD dec reased (P< 0.01) and MDA increased (P< 0.01) after reperfusion in group NPY; ③ Comparing with group MIR+NPY, NO, SOD increased (P< 0.01) but MDA decreased (P< 0.01) in group MIR+NPY+PAMD. The difference of NO , SOD, MDA was insignificant (P> 0.05) comparing with those in group MIR . Conclusion: NPY may make myocardial ischemia reperfusion injury more serious by the way of dysfunction of endothelia and PAMD may revers e that influence and protect endothelium function and reduce myocardia ischemia reperfusion injury in rabbits.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2001年第9期407-408,412,共3页
Journal of Clinical Cardiology
关键词
神经肽Y
蝙蝠葛酚性碱
内皮功能
心肌缺血
再灌注损伤
MIR
Neuropeptide Y
Phenolic alkaloids of menispe rmum dauricum
Myocardial reperfusion
Endothelium function
