摘要
目的 :体外培养条件下观察内毒素对大鼠肝巨噬细胞 (Kupffer cells,KC)谷氨酰胺代谢的影响。 方法 :分离、纯化获得的大鼠 KC体外培养 ,观察在不同浓度内毒素时 ,KC对谷氨酰胺 (Gln)消耗、利用率和细胞内 Gln浓度的影响。 结果 :培养液中不含内毒素时 ,KC对 Gln的消耗、利用率和细胞内含量分别为 (2 2 5± 2 4)μmol/ L、(9.33± 0 .99) %和 (142 5± 2 0 1)μmol/ 1.5× 10 6 KC。内毒素浓度为 10 mg/ L ,其 Gln的消耗、利用率和细胞内含量最大值分别为 (4937± 2 89)μmol/ L、(73.2 9± 4.72 ) %和 (5 117± 389)μmol/ 1.5× 10 6 KC。 结论 :Gln是静息状态和内毒素激活时 KC的重要代谢底物。
Objectives:To observe the metabolism of glutamine in the rat Kupffer cells and the effect of endotoxin. Methods:The changes of glutamine concentrations were measured before and 24 h after the in vitro cultures of the rat Kupffer cells with or without the stimulations of lipopoly saccharide. Results:Consumption and utilization rate of Gln in Kupffer cells with no LPS were (225±24)μmol/L and (9 33±0 99)% repectively.When the concention of LPS was 10.00 mg/L,consumptoin and utilization of Gln were (4 937±289)μmol/L and (73.29± 4.72 )%. Conclusions:Glutamine is an important substrate of Kupffer cell metabolism in the rest and LPS activated conditions.
出处
《肠外与肠内营养》
CAS
2001年第3期155-156,共2页
Parenteral & Enteral Nutrition
