摘要
目的 测定增生性瘢痕和瘢痕疙瘩等瘢痕组织中PKC活性变化 ,探讨PKC在瘢痕形成中的作用。方法 利用PKC活化后催化底物多肽磷酸化 ,然后计数底物中掺入32 P的放射活性的原理 ,测定增生性瘢痕、瘢痕疙瘩、成熟瘢痕和正常皮肤组织中PKC活性。结果 增生性瘢痕和瘢痕疙瘩组织中PKC的活性显著高于成熟瘢痕和正常皮肤 (P <0 .0 0 1,分别高出正常皮肤 318.30 %和 5 19.0 7% ) ,瘢痕疙瘩高于增生性瘢痕 (P <0 .0 0 1,高出 48.6 0 % )而成熟瘢痕和正常皮肤间没有差异 (P >0 .0 5 )。结论 瘢痕组织中PKC活性升高且与增生程度相关 。
Objective To determine the activity of protein kinase C(PKC) in hypertrophic scar(HS) and keloid(K) tissue and to study the effects of PKC on scar formation. Methods The principle of activated-PKC to transfer phosphorus ( 32 P) into substrate was used to calculate activity of PKC by detecting radioactivity of substrate with scintillation counter. Six cases of HS and K were studied. Mature scar (MS) and normal skin (NS) were used as control. Results Activity of PKC in HS and K was obviously elevated as compared with that in MS and NS (P<0.001): and in keloid its activity was higher than in prolifenative scar. The difference between MS and NS was insignificant (P>0.05). Conclusion Activity of PKC in scar tissue is elevated and increasing degree of PKC may be related to proliferative level of scar.
出处
《实用美容整形外科杂志》
2001年第3期149-151,共3页
Journal of Practical Aesthetic and Plastic Surgery
基金
全军杰出人才基金资助
