摘要
目的比较体外培养正常成年大鼠、大鼠乳鼠及人胎肝细胞的生物学性能。方法采用不同的分离方法获取肝细胞进行体外培养 ,台盼蓝染色法测定细胞活率 ,ＭＴＴ法检测细胞增殖活力 ,通过检测白蛋白分泌反映肝细胞的功能。结果人胎肝细胞生长活力和蛋白合成功能最佳 ,乳鼠肝细胞次之 ;乳鼠肝细胞较人胎肝细胞易传代 ;成年大鼠肝细胞分离操作方便 ,但在体外存活时间较短。结论不同取材来源的肝细胞体外培养性能有显著性异 (Ｐ < 0 . 0 5)。
ObjectiveTo compare the biological function of cultured hepatocytes in vitro from different origins, including normal adult rats, new born rats and human fetal liver.MethodsHepatocytes were obtained and cultured in vitro with different isolating methods. The viable rate was assessed by typan blue exclusion test, the proliferating viability was evaluated by MTT method and the albumin was detected to reflect the function of hepatocytes.ResultsThe human fetal hepatocytes were of better viability and protein secreting function in vitro than those of new born rats.However,the latter was subcultured more easily than the former.The hepatocytes from adult rats,which were isolated conveniently,lived the shortest life time in vitro.ConclusionThe biological function of cultured hepatocytes in vitro from different origins is significantly different (P< 0.05 ). MeSH liver;cells,cultured;albumins;in vitro;embryo;rat
出处
《河北医科大学学报》
CAS
2001年第5期269-272,共4页
Journal of Hebei Medical University
关键词
肝细胞
白蛋白
体外培养
胚胎
大鼠
liver
cells,cultured
albumins
in vitro
embryo
rat
