摘要
本文采用反相高效液相色谱法测定海水中溶解态游离氨基酸,整个分析过程只需40 min就可使所有海水样品中的氨基酸得到很好分离,对各含6pmol的16种氨基酸混合样分析偏差在10%左右,检测极限为100fmo1,线性范围在1pmol到Inmol之间。
This paper describes a method of reversephase high performance liquid chromatohraphy for the determination of dissolved free amino acids in seawater.The whole process takes only 40 minutes to separate the amino-acids in all the sea-water samples. The coefficient of variation of 16 kinds of amino-acids samples each containing 6 p mol is about 10%, the detection is 100 f mol, the linear range is 1p mol to 1n mol.
出处
《海洋科学》
CAS
CSCD
北大核心
1989年第2期43-47,共5页
Marine Sciences
关键词
HPLC法
海水
氨基酸
溶解态
Free amino acids, Reversed-phase high performance liquid chromatograhy (RP-HPLC), Ophthaldialdehyde (OPA)
