摘要
目的 探讨锌离子对雷公藤多甙诱导人前骨髓白血病细胞 (HL 6 0 )生物周期变化的影响。方法 ①测定HL 6 0的细胞表型、细胞膜Fas蛋白的表达 ;②将HL 6 0与雷公藤多甙共培养 ,观察细胞形态、生物周期变化和DNA电泳分析以及锌离子对细胞生物周期变化的影响。结果 ①HL 6 0细胞表型为CD4+CD8-CD19-IFN γ+IL 8-,细胞膜Fas蛋白表达较弱 ;②与雷公藤多甙共培养后 ,HL 6 0发生了与细胞凋亡相关的细胞生物周期变化 ;此作用主要影响G2 /M和S期细胞 ,并且呈剂量和时间依赖性 ;③锌离子几乎完全阻断雷公藤多甙诱导HL 6 0细胞生物周期变化的作用。结论 ①HL 6 0具有Th1淋巴细胞样细胞特征 ,Fas膜蛋白表达较弱 ;②雷公藤多甙诱导HL 6 0发生的细胞周期变化受锌离子影响。推测 :在体内 ,锌离子可能影响雷公藤多甙对多种自身免疫性疾病的临床治疗作用及副作用。
Objective To explore the effect of Zn 2+ on human leukemia promyelocyte (HL 60)cycle changes induced by multiglucoside tripterygii (MT).Methods ① HL 60 phenotype and membrane protein Fas expression was detected.② HL 60 was cultured with MT observing the cell forms and cell cycle changes,DNA electrophoresis results and the effect of Zn 2+ on the cell cycle change.Results ① The HL 60 were CD4 +CD8 -CD19 -IFN γ +IL 8 -,and the Fas protein expression was poorer.② Following culture of HL 60 with MT,the apoptosis relative cell cycle change appeared in HL 60.This change mainly affected G 2/M and S stage cells,and presented dose time correlation.③ Zn + nearly all blooked the effect of MT induced HL 60 cell cycle change.Conclusion ① HL 60 has Th1 lymphocyte like characteristics,and the Fas protein expression is poorer.② Zn + affects the MT induced HL 60 cell cycle change.It suggests that Zn + might affect the therapeutic and side effects during clinical treatment of many autoimmune diseases with MT.
出处
《中华风湿病学杂志》
CAS
CSCD
2001年第4期230-233,共4页
Chinese Journal of Rheumatology
