摘要
利用小鼠免疫保护模型观察了福氏2a痢疾菌(F_(2a)-12)、宋内氏痢疾菌(48025)及其变异株(F_(2a)-12A、宋R)全膜的免疫保护作用,分析了起免疫保护作用的因子。以不同型痢疾菌的全膜皮下免疫小鼠,用相应有毒菌株攻击,结果表达脂多糖(LPS)抗原和毒力相关蛋白抗原的宋内氏菌48025对小鼠有同型保护作用,保护率为100％;宋R不表达LPS和毒力相关抗原则无保护作用;表达毒力相关蛋白抗原和LPS的F_(2a)-12和不表达毒力相关蛋白抗原但表达LPS的F_(2a)-12A均对小鼠有同型保护作用,保护率达90％～100％。本文对福氏2a痢疾菌,宋内氏痢疾菌及其变异株的主要抗原成分与免疫保护作用的关系进行了讨论。
The immunoprotective effects of membrane fractions from ShigeUa flexneri 2a, S.sonnei and their mutants (F_(2a)-12A, S.sonnei-R) were observed on mice. Mice were immunized subcutaneously with membrane fractions from various strains of shigella and challenged intraperitoneally with homologous virulent strains. Results showed that both virulent S. flexneri 2a and S. sonnei could protect mice against homologous challenges. S. sonnei-R which lost Oantigen and virulence-associated proteins has no protective effect, but the mutant F_(2a)-12A which only lost virulence-associated proteins could afford protection to mice. The relation between membrane components and immunoprotection were discussed.
出处
《军事医学科学院院刊》
CSCD
北大核心
1991年第3期169-172,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
痢疾杆菌
变异株
免疫保护
Shigella
mutants
strains
virulence-associated protein antigen
LPS antigen
immunoprotection
