摘要
枯萎病菌侵染后 ,抗病品种棉苗及经氟乐灵诱发处理产生对枯萎病诱导抗性的棉苗茎与根组织中 ,膜脂过氧化产物丙二醛 ( MDA)含量上升 ,膜系统中不饱和脂肪酸含量下降 ,表明有膜脂过氧化的发生 ,超氧阴离子 ( O-·2 )的产生速率、H2 O2 的积累水平及脂氧合酶 ( L OX)活性也明显增加 ,但超氧化物歧化酶 ( SOD)及过氧化氢酶 ( CAT)活性无显著差异。与此相反 ,感病品种棉苗受侵后 MDA含量、O-·2 产生速率、H2 O2 的积累及 LOX酶活性增加幅度较小 ,SOD及 CAT酶活性则明显上升 ,膜系统中不饱和脂肪酸含量仅有小幅度下降。氟乐灵播前土壤处理可诱发棉苗对枯萎病的诱导抗性 ,同时也增加了 LOX酶活性及活性氧 ( O-·2 、H2 O2 )的积累。这些结果说明 ,枯萎病菌侵染后棉苗体内活性氧的积累、LOX酶活性的上升以及由此引起的膜脂过氧化 。
Seeds of cotton ( Gossypium hirsutum ), either resistant or susceptible cultivar to Fusarium wilt, were sown in soil treated with trifluralin at 2 μg a.i./g soil. The 10 day old seedlings were inoculated with Fusarium oxysporum f.sp. vasinfectum (Fov) . Active oxygen species and lipid peroxidation levels in the seedling stems/roots were determined to evaluate their relationship to cotton resistance against the disease. The levels of malondialdehyde (MDA) and the content of unsaturated fatty acid in membrane significantly increased after the inoculation in the seedlings of resistant cultivar, especially in the trifluralin-treated seedlings, showing the enhanced lipid peroxidation. Moreover, the generation rate of superoxide anion (O [SX(B-3]-· 2), the level of hydrogen peroxide (H 2O 2) and the activity of lipoxygenase (LOX) were also increased markedly in the seedlings mentioned above. But no significant increases in the activities of superoxide dismutase (SOD) and catalase (CAT) were observed in those seedlings. In contrast, the MDA level,the production rate of O [SX(B-3]-· 2,the content of H 2O 2,and LOX activity in cotton seedlings of susceptible cultivar increased slightly. The content of unsaturated fatty acid in membrane decreased slightly after infection by Fov ,while the activities of SOD and CAT increased significantly. The trifluralin treatment, showing effect on inducing cotton resistance to Fusarium wilt,increased the LOX activity,the contents of O [SX(B-3]-· 2 and H 2O 2,and MDA level. The results suggested that the accumulation of active oxygen species (O [SX(B-3]-· 2 and H 2O 2) and the increased activity of LOX may play a role in the resistance of cotton seedlings to Fusarium wilt by initiation of lipid peroxidation.
出处
《植物病理学报》
CAS
CSCD
北大核心
2001年第2期110-116,共7页
Acta Phytopathologica Sinica
基金
浙江省自然科学基金资助项目! (392 16 6 )
关键词
棉花
棉花枯萎病菌
膜脂过氧化
活性氧
抗病性
Gossypium hirsutum
Fusarium oxysporum f.sp. vasinfectum
lipid peroxidation
active oxygen species
resistance
