摘要
目的 :研究脑创伤后大鼠神经元内游离钙 ([Ca2 + ] i)及微管相关蛋白 2 (MAP2 )的变化 ,探讨脑创伤后神经元内 [Ca2 + ] i 与MAP2的关系。方法 :利用激光共聚焦显微镜测定脑创伤后神经元内 [Ca2 + ] i及MAP2的变化。结果 :尼莫地平于伤后 1h内应用可减轻MAP2的丢失 ,而伤后 10h以上应用则无效。尼莫地平于伤后 2 2h内应用均可降低神经元内 [Ca2 + ] i升高。结论 :尼莫地平可降低伤后神经元内 [Ca2 + ] i升高及MAP2丢失 ,但应用的有效时间窗不同 ,提示脑创伤后神经元内 [Ca2 + ] i
Aim:To investigate alteration in [Ca 2+ ] i and microtubule associated protein 2 (MAP2) in Wistar rat's neuron following fluid percussing injury (FPI) and relationship between neuronal [Ca 2+ ] i and MAP2 following FPI. Methods:Alteration in [Ca 2+ ] i and MAP2 in Wistar rat's neuron following FPI was measured with a confocal laser scanning microscope. Results:Application of Nimodipine markedly reduced the loss of MAP2 immunoreactivity within 1 h postinjury ( P <0 01), but application of it had no effect beyond 1 h postinjury. Application of Nimodipine following FPI decreased the increase of [Ca 2+ ] i in neuron following FPI within 22 h postinjury. Conclusion:Nimodipine reduced the increase of [Ca 2+ ] i and the loss of MAP2 in neuron following FPI, but application of it had different time windows following FPI. The result indicated that the increase of neuronal [Ca 2+ ] i following FPI was not a single reason of degradation of MAP2.
出处
《中国临床神经科学》
2001年第2期136-138,共3页
Chinese Journal of Clinical Neurosciences
关键词
脑创伤
游离钙
尼莫地平
微管相关蛋白2
fluid percussing brain injury free calcium iron Nimodipine microtubule associated protein 2
