摘要
目的 研究BcL 2、p5 3表达在胃癌发生、发展中的作用及二者的相关性。方法 采用免疫组化双重染色法检测了 15 6例正常胃粘膜、不典型增生及胃癌组织中BcL 2和p5 3的表达。结果 BcL 2和p5 3在正常胃粘膜中均呈阴性表达。BcL 2在重度不典型增生和高分化腺癌中表达率较高 (分别为 6 9 6 %和 73 9% ) ,与轻度不典型增生及中低分化腺癌间差异显著 (P <0 .0 5 )。p5 3在胃癌中的表达显著高于胃粘膜不典型增生 ,且表达率随着胃癌分化程度的降低而升高 (P <0 .0 5 )。胃癌淋巴细胞转移组 ,BcL 2蛋白低表达 ,p5 3蛋白高表达 (P <0 .0 5 )。BcL 2与p5 3呈显著负相关 (P <0 .0 1)。结论 BcL 2与p5 3分别作用于胃癌发生、发展的不同阶段 。
Objective To study the role and their correlationship of BcL 2 and p53 expression in gastric carcinogenesis.Methods Immunohistochemical double staining technique was used to detect the expression of BcL 2 and p53 in 156 cases of gastric specimens.Results Both BcL 2 and p53 were negatively expressed in normal gastric mucosa.A high positive rate of BcL 2 in severe atypical hyperplasia (69.6%) and well differentiated adenocarcinoma (73.9%) were found,and the positive rate was significantly higher than that in minor atypical hyperplasia and moderate differentiated and poor differentiated adenocarcinoma( P <0.05).The expression of p53 in gastric carcinoma was significantly higher than that in gastric mucosa atypical hyperplasia and elevated with low degree of differentiation in gastric carcinoma( P <0.05).In gastric carcinoma with lymphatic metastasis,it showed low expression of BcL 2 and high expression of p53( P <0.05).There was significantly reverse relevant between p53 and BcL 2 proteins expression( P <0.01).Conclusion BcL 2 and p53 play the roles in different stage of carcinogenesis and development respectively and contribute to early diagnosis and evaluating prognosis of gastric carcinoma.
出处
《哈尔滨医科大学学报》
CAS
2001年第3期173-175,共3页
Journal of Harbin Medical University
