摘要
采用正相高效液相色谱法，梯度洗脱程序和蒸发光散射检测器对大豆磷脂组成进行分析，在15 min内将大豆磷脂中4种重要组分：卵磷脂、脑磷脂、肌醇磷脂和磷脂酸与其它组分完全分离。用外标法对这4 种重要成分进行定量，线性范围为0.2～５.8 g/L；回收率为96.7～100.8%；相对标准偏差为0.82%～1.34%。方法用于实际样品测定，获得满意的结果。
Soybean phospholipids were analyzed by positive-phase high performance liquid chromatography using gradient eluted program and evaporative light-scattering detector. The four important components, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidyl acid of soybean phospholipids were separated completely with other components in fifteen minutes. The quantitative analysis of the four important components has been achieved with external standard method. The linear range is 0.2 similar to 5.8 g/L. The recovery of the method is 96.7% similar to 100.8%, the relative standard deviations is 0.82% similar to 1.34%. This method has been applied to the analysis of soybean phospholipids.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第9期1046-1048,共3页
Chinese Journal of Analytical Chemistry
关键词
高效液相色谱
大豆磷脂
卵磷脂
脑磷脂
分析
high performance liquid chromatography
soybean phospholipid
phosphatidyl choline
phosphatidyl ethanolamine
