摘要
基于分子间静电相互作用力 ,将锇 -聚乙烯吡啶复合物 ( PVP-Os)与辣根过氧化物酶 ( HRP)交替沉积于固体基质表面 ,制得了包含生物成分的分子多层膜 .膜层间的聚合物分子起到了粘接与导电的双重作用 .用紫外 -可见光谱法跟踪了石英基片上的组装过程 ,研究了多层膜电极对过氧化氢的电催化还原性能 。
We prepared a new kind of multilayer films based on ionic self assembly technique by successive alternating deposition of positively charged polycation bearing Os complex(PVP Os) and horseradish peroxidase(HRP) on the surface modified of gold electrode to study the electrochmical behavior and quartz slides to monitor the assembly process by ultraviolet visible spectroscopy. The electrochemical behavior of the enzyme electrode showed that the multilayer films were stable, electroactive, the mediator PVP Os as electron shuttle was able to transfer electrons successfully between the active center of the immobilized HRP and the electrode surface.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2001年第9期1601-1604,共4页
Chemical Journal of Chinese Universities
基金
吉林省自然科学基金资助
