摘要
从 2 0余株酵母菌中筛选得到可转化D 木糖生产木糖醇的热带假丝酵母。以此为出发菌株 ,通过UV重复诱变 ,并结合高糖平板分离 ,得到木糖还原酶活力较高的较佳诱变株ZG 2 4。当初糖浓度为85 3 6g/L时 ,经 1 0L罐发酵 64h,木糖醇含量达 60 87g/L ,木糖利用率为 94 1 1 % ,产物木糖醇得率达 75 78%。
A Candida tropicalis strain that bioconverting D-xylose t o xylitol was s creened out from more than 20 yeastsStrain ZG-24,with high D-xylose reducta se activity,was obtained by UV repeated mutation and selection with high xylose c oncentration plateFermented for 64 h with 8536 g/L initial xylose content in 10 L fermentor,the xylitol content was 6087g/L,utilization ratio of xylose w as 9411%,with 7578% xylitol yieldTime curves of D-xylose reductase and x ylitol dehydrogenase were also investigated
出处
《药物生物技术》
CAS
CSCD
2001年第3期156-159,共4页
Pharmaceutical Biotechnology
基金
浙江省科委资助项目 (编号 :97110 3 13 8)
