摘要
为提高草鱼对出血病的抗性 ,采用显微注射法 ,将克隆在鲤 β-肌动蛋白基因启动子下游的人 α-干扰素基因转移到草鱼受精卵中 ,获得了大量转基因个体 .抽取转基因鱼血浆 ,以酶联免疫吸附法检测了转基因草鱼中人α-干扰素基因的表达情况 .从 6 72尾转基因草鱼中检测出 15 8尾有 α-干扰素的表达 ,阳性率为 2 3.5 1% ,其中表达水平最高个体血浆中α-干扰素质量浓度为 145 0 pg/ m L .
To raise carp's resistance to hemorrhage,a large amount of transgenic grass carp was obtained by transfering hu IFN α gene which was cloned under the control of the β actin gene promoter of grass carp to the cytula of carp in the way of microinjection. ELISA detection of the gene expression of the transgenic grass carps was carried out by using the serum as samples.158 individuals were tested to be positive with a detectable hu IFN α expression level in 672 transgenic fish. The detectable ratio is 23.51% and the highest is 1 450 pg/mL in serum. The expression level of the transformed gene changes in different seasons and development stages.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第3期177-178,共2页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家"8 6 3"计划资助项目 (10 1- 0 1- 0 5 )
