摘要
目的：以柯萨奇病毒Ｂ３（ＣＶＢ３）感染体外培养的小鼠心肌细胞，探讨ＣＶＢ３感染心肌细胞后对心肌细胞周期、心肌细胞凋亡和心肌细胞浆游离钙的影响。 方法：制备心肌细胞样本，设正常对照组及实验组（ＣＶＢ３感染心肌细胞），流式细胞仪、钙荧光探针标记分别检测细胞周期、细胞凋亡及心肌细胞浆内游离钙。 结果：ＣＶＢ３感染心肌细胞后，可显著抑制心肌细胞的分裂增殖，诱导心肌细胞凋亡；显著增高心肌细胞浆内游离钙浓度和减少细胞外钙含量。 结论：ＣＶＢ３感染体外培养的心肌细胞，可能引起心肌细胞凋亡和心肌细胞浆内钙超载，初步实验提示此种变化可能是病毒性心肌炎心脏损伤的重要机制。
Objective:The mouse cardiomyoc ytes cultured in vitro were infected wit h Cosackievirus B3(CVB3)for the investi gation of the cell cycle,apoptosis and c ytoplasmic free calcium level in the CVB 3-infected cardiomyocytes. Methods:Cell culture,flow cytometery, calcium fluorescent probing and atom absor ption spectrophotometery Results:The CVB3-infected cardiomyocy tes showed significant inhibition on the division and proliferation,induction of cell apoptosis,apparent increase in the intracellular free calcium and decrease in the extracellular calcium level. Conclusions:Apoptosis and intracellular calci um overload of the CVB3 infected cardio myocytes are the key mechanism underlyin g the cardiac lesion in the case of vira l myocarditis.
出处
《中国循环杂志》
CSCD
北大核心
2001年第4期299-302,共4页
Chinese Circulation Journal
