摘要
目的 观察IL 2、4、6、7和IFN γ对γ射线诱导的人扁桃体淋巴细胞凋亡的调节作用。方法 取手术切除的扁桃体组织 ,经Ficoll分离得单个核细胞 ,采用 4Gy6 0 Coγ射线照射 ,用凝胶电泳显示细胞凋亡 ;体外用IL 2、IL 4、IL 6、IL 7及IFN γ与单核细胞共培养 2 4h后流式细胞仪测CD4+ 、CD8+ T细胞及CD19+ B细胞的凋亡率及表面bcl 2的表达。结果 照射后 4～ 2 4h有明显的梯形条带出现 ;CD8+ T细胞和B细胞的辐射敏感性较CD4+ T细胞高 ;IL 2、IL 4和IL 7对CD4+ 、CD8+ T细胞凋亡及IL 4对B细胞凋亡有明显的保护作用 ,同时可观察到bcl 2明显的高表达。IL 6和IFN γ无此作用。结论 细胞因子IL 2、IL 4与IL 7可促进放射病病人免疫功能恢复。
Objective\ To observe the protective action of IL\|2,4,6,7 and IFN\|γ on radiation\|induced apoptosis of human tonsil lymphocyte subpopulation. Methods\ Human tonsil specimens were obtained from routine tonsillectomies.Lymphocytes were separated and co\|cultured with IL\|2,4,6,7 and IFN\|γ for 24 h,then exposed to 4 Gy γ\|rays.DNA gel electrophoresis and flow cytometric light scatter analysis were performed to discriminated the viable cells from dead cells,and bcl\|2 expressed on it. Results\ The “ladder pattern” of DNA fragmentation could be seen within 4-24 h after 4 Gy irradiation.CD8 +T and B cells showed higher radiosensitivity than that of CD4 + T cells.The radiation\|induced apoptosis in both CD4 + and CD8 + T cells was significantly inhibited and bcl\|2 expression was induced by IL\|2,IL\|4 and IL\|7.The viability of B cells was maintained and bcl\|2 was overexpressed by the presence of IL\|4 but not by others in culture. Conclusion\ We conclude that the protective effect by each cytokine on radiation\|induced apoptosis might be partly attributed to their enhancement of cellular expression of bcl\|2 protein.\;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2001年第4期297-300,共4页
Chinese Journal of Radiological Medicine and Protection
基金
总装备部医药卫生重点科研课题基金资助项目(2 990 0 4)
